Calcium L-5-Methyltetrahydrofolate
Bulk Calcium L-5-Methyltetrahydrofolate for Your Supplement Products
- CAS Number: 151533-22-1
- EINECS Number: 691-636-3
- Molecular Weight: 497.53
- Molecular Formula: C20H23CaN7O6
- INN Name: Levomefolate Calcium
- Related Name: L-5-MTHF-Ca, L-5-Methyltetrahydrofolate Calcium, L-Methylfolate Calcium, 5-MTHF-Ca, Active Folate Calcium, Bioactive Folate, The 4th Generation Folate
What Is Calcium L-5-Methyltetrahydrofolate?
Calcium L-5-Methyltetrahydrofolate is an active coenzyme form of folate, commonly listed on premium labels as folate as L-methylfolate. L-5-MTHF-Ca is active and does not need to undergo a multi-step enzymatic conversion by the liver. It directly participates in cellular methylation as a fully bioidentical, active folate. The stable structure ensures rapid systemic absorption and blood-brain barrier (BBB) delivery. In this way, it supports cellular and cognitive functions.
Functions of Our Calcium L-5-Methyltetrahydrofolate
Elevated homocysteine levels are dangerous factors that may cause cardiovascular diseases. Active folate is the primary methyl donor. It can efficiently transfer homocysteine to methionine. In this way, it reduces the serum homocysteine levels and protects the vascular endothelium.
You can apply it in your vascular health supplements and aim at senior populations and people who have sleeping or alcohol issues.
Active folate can cross the blood-brain barrier and directly participate in the generation of essential neurotransmitters, such as serotonin, dopamine, and norepinephrine. It helps adjust moods, mitigate anxiety, and manage depression as a complementary product. You can use it in our mood regulation supplements.
Folate is necessary in the process of cellular division and DNA synthesis. As an active form of folate, Calcium L-5-Methyltetrahydrofolate is ideal for pregnant women. It can significantly reduce the risk of fetal neural tube defects (NTDs), such as spina bifida.
You can highlight that it provides safe and reliable protection for pregnant women and fetuses when promoting your supplements.
Calcium L-5-Methyltetrahydrofolate supports red blood cell production by stimulating the proper division and maturation in the bone marrow. In this way, it prevents megaloblastic anemia caused by a lack of intracellular folate.
We suggest marketing your final product with the function of supporting healthy blood formation.
More About Our Company
- Professional Calcium L-5-Methyltetrahydrofolate Supplier
- Features
- Manufacturing
- Specification
- Over 10 years of experience in manufacturing Calcium L-5-Methyltetrahydrofolate
- Partner with global customers from more than 50 countries
- Strict quality control to meet overseas market requirements
- Minimized heavy metal content and other residues
- Stable supply chain for bulk orders in the long term
Active Folate Form: A large portion of the worldwide population has MTHFR genetic mutations. They have reduced efficiency in converting folic acid into its active form. Sunjoy produces active folate that the human body can absorb directly.
Zero Unmetabolized Residue: Sunjoy L-5-MTHF-Ca is aligned with the body’s endogenous metabolic pathways. It helps avoid the conversion limitations associated with unmetabolized folic acid and ensures an exceptional safety profile without the risk of UMFA accumulation.
High Bioavailability and Exceptional Shelf-Life: The specific form of calcium salt significantly improves the chemical stability. Our powder is resistant to environmental degradation and has a long shelf life.
Excellent Dosage Compatibility: Sunjoy active folate has a high compatibility with different forms of supplements (including capsules, softgels, tablets) and many other ingredients in the formula.
Step 1: Raw Material Inspection and Preparation: We inspect and accurately weigh all the raw materials (including Folic Acid (FA), sodium dithionite, and zinc powder). We ensure the ingredients are controlled under strict anaerobic production conditions before the reaction sequence.
Step 2: Chemical Reduction to Dihydrofolate (DHF):
Folic Acid (FA) possesses an initial chemical reduction mediated by Zinc powder and sodium dithionite. In this procedure, the specific double bonds are broken down and efficiently converted into Dihydrofolate (DHF).
Step 3: Enzyme-Catalyzed Conversion to Tetrahydrofolate (THF)
Under the strictly controlled temperature and pH conditions, we use Dihydrofolate Reductase (DHFR) to convert DHF into Tetrahydrofolate (THF). This process efficiently reduces unwanted isomers.
Step 4: Methylation and Sodium Borohydride Reduction
The intermediate solution of Tetrahydrofolate (THF) reacts with a methylating agent.
Then, under the accurate reaction parameter, we use sodium borohydride to further reduce it and generate the crude solution of L-5-Methyltetrahydrofolic acid.
Step 5: Centrifugation and Calcium Salification
We apply centrifugation to the crude solution to remove zinc residues and enzyme proteins. Then, we add a pharmaceutical-grade calcium source under stable pH conditions to precipitate stable Calcium L-5-Methyltetrahydrofolate.
Step 6: Chromatographic Purification and Decolorization
The salified mixture undergoes advanced chromatographic columns to eliminate the residual substrates. Then it goes through a heated activated carbon treatment to remove the trace amount of pigments and organic impurities. After these processes, the chemical and optical purity is maximized.
Step 7: Crystallization, Drying, and Quality Inspection
The purified solution is processed through strictly controlled cooling crystallization and mild vacuum drying to get the stable crystals. Then they undergo the final comprehensive physicochemical and microbiological testing to ensure the quality of our product.
| Process Control and Quality Assurance Supplement Table | |||
| Key Process Stage | Process Objective | Potential Risk | Quality Control Measure |
| Raw Material Inspection | Ensure qualified starting materials | Raw material variability or impurities | Identity verification and incoming quality inspection |
| Chemical Reduction (DHF) | High conversion efficiency to DHF | Incomplete reduction or degradation | Precise Na2S2O4 / Zn dosage and nitrogen protection |
| Enzymatic Reaction (THF) | High stereoselective conversion | Enzyme inactivation or loss of selectivity | Controlled temperature, pH, reaction time, and enzyme dosage |
| Methylation and Reduction | Form L-5-Methyltetrahydrofolate | Incomplete methylation or over-reduction | Controlled NaBH4 addition rate and strict temperature monitoring |
| Centrifugation and Salification | Form stable Calcium salt crystals | Residual zinc/proteins or poor salt conversion | Optimized centrifugation parameters and precise calcium source dosing |
| Purification and Decolorization | Maximize product chemical purity | Residual reaction by-products or discoloration | Multi-stage chromatographic separation and controlled carbon dosage |
| Crystallization, Drying, and QC | Produce qualified L-5-MTHF-Ca powder | Irregular crystal form or specification deviation | Controlled cooling curves, vacuum drying, and HPLC testing |
| Calcium L-5-Methyltetrahydrofolate COA | |
| Contents | Standards |
| Appearance | This product is a white to light yellow crystalline powder with no visible impurities and no odor. |
| MOQs | 25 kg/ drum |
| Sample | Available (10-20 g/ bag) |
| Identification | |
| 1. IR | The infrared absorption spectrum of this product should be consistent with the absorption spectrum of the standard product. |
| 2. HPLC | In the chromatogram obtained under the content determination item, the retention time of the main peak of the test solution is consistent with the retention time of the main peak of the reference solution. |
| 3. Calcium | Should comply with the calcium salt identification reaction. |
| D-5-Methylfolate | ≤1.0% |
| Assay (On Anhydrous Basis) | 95.0%~102.0% |
| Water | 6.0%~17.0% |
| Calcium (On dry basis) | 7.0%~8.5% |
| Chloride | ≤0.5% |
| Residual Solvents | Ethanol ≤5,000 ppm Isopropyl alcohol ≤5,000 ppm |
| Elemental Impurities | |
| Boron | ≤50mg/kg |
| Platinum | ≤10mg/kg |
| Arsenic | ≤1.5mg/kg |
| Lead | ≤1.0mg/kg |
| Cadmium | ≤0.5mg/kg |
| Mercury | ≤1.5mg/kg |
| Related Substances | |
| Impurity A | ≤0.5% |
| Impurity B | ≤1.0% |
| Impurity C+D | ≤1.0% |
| Impurity E | ≤0.5% |
| Impurity F | ≤0.5% |
| Impurity G | ≤0.5% |
| Impurity H | ≤0.5% |
| Impurity I | ≤0.5% |
Related Products
Pyridoxal-5'-Phosphate PowderActive folate and active vitamin B6 are a synergistic duo for methylation. Calcium L-5-Methyltetrahydrofolate provides the methyl group for the methionine cycle. Pyridoxal-5′-Phosphate promotes the conversion of homocysteine into cystathionine and prevents toxic accumulation.
You can apply them in your nutritional supplements, such as premium cardiovascular formulas, and highlight the cardiovascular protection in the promotions.
Active folate and vitamin B2 are a fundamental metabolic duo. Calcium L-5 Methyltetrahydrofolate directly delivers the active and absorbable form of folate to the cells. Riboflavin stimulates the MTHFR enzyme in the human body. It triggers the internal process to make sure folate continuously has its effects. This collaborative mechanism maximizes the absorption and utilization rate greatly.
You can use them in your health products, such as MTHFR support formulas.
Pyridoxamine DihydrochlorideFolate and Vitamin B6 are a powerful anti-aging combo. L-5-MTHF-Ca supports DNA repair and cellular renewal. Pyridoxamine Dihydrochloride blocks the accumulation of advanced glycation end-products (AGEs). They can boost cellular energy and reduce the influence of daily metabolic aging.
You can combine them in your advanced anti-aging formulations. They are ideal for longevity nutrition complexes and cognitive support supplements.
Calcium L-5-Methyltetrahydrofolate can promote cognitive focus and maintain the nervous system healthy. Thiamine Nitrate converts carbohydrates into ATP energy. In this way, it offers fuel for the brain cells.
You can apply them in your premium brain performance formulations. They are suitable for brain health supplements, stress management formulas, and Vitamin B complexes for pregnant women.
Our standard MOQ is 25kg for trial orders. We are fully capable of producing long-term bulk orders reaching metric tons of our product. We also offer 10g to 20g free samples for lab testing. We pack the samples in Aluminum foil bags and pack bulk powder in cardboard drums with food-grade PE bags inside.
In most cases, it is safe to take Calcium L-5-Methyltetrahydrofolate supplements. As a natural active form of folate, it bypasses the body’s complex conversion process. Therefore, it rarely causes side effects. In specific cases, the following mild side effects may appear:
Mild Digestive Discomfort
Some people may experience mild discomfort in the stomach, nausea, or minor bloating when they take active folate for the first time. Due to the high bioavailability, the discomfort in the digestive system is significantly reduced compared to conventional synthetic folic acid.
Mild Physiological Reactions
Minor side effects may include a temporary mild headache, dizziness, and a brief shift in energy levels. These symptoms are very rare and mild. They usually disappear as the body gradually adapts to active folate.
Mild Stimulation or Restlessness (Rare)
A very small percentage of users may feel slightly anxious, restless, or have vivid dreams. These symptoms are quite rare, especially when compared to the raw, uncomplexed folate derivatives.
You should keep Calcium L-5-Methyltetrahydrofolate powder in a dry and cool place, and keep it away from direct sunlight. Under proper storage conditions, Sunjoy active folate has a long shelf life of up to 3 years.
Yes, we do. Sunjoy is dedicated to supporting your brands in developing international markets. We are familiar with global regulatory requirements. And our technical team will offer you standard technical dossiers. We will help your final products meet the local compliance requirements smoothly.
| Supported Documentation & Certifications | ||
| Target Market / Region | Regulations & Standards | Available Documents |
| Global / General | ISO, cGMP, HACCP | COA, MSDS, Specification Sheet, Manufacturing Flow Chart |
| USA | FDA, GRAS | US FDA Facility Registration, DMF (Drug Master File) under preparation |
| Europe | EFSA, Novel Food | TSE/BSE Free Certificate, Non-GMO Statement, Allergen Statement |
| Global Religious | Halal & Kosher | Halal Certificate, Kosher Certificate |
If you need more information, feel free to contact our service team.
Contact Us
If you are searching for bulk Calcium L-5-Methyltetrahydrofolate for your supplements, Sunjoy meets your demands with advanced equipment and modern production lines. We manufacture pure active folate calcium powder and deliver on schedule. Contact us and get your Calcium L-5-Methyltetrahydrofolate soon.
